crispr-cas9 crrna (Integrated DNA Technologies)
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Integrated DNA Technologies
crispr-cas9 crrna
Crispr Cas9 Crrna, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 95/100, based on 744 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9/CRISPR-Cas9+crRNA/custom%40crispr-cas9-crrna%4042951897
Average 95 stars, based on 744 article reviews
Crispr Cas9 Crrna, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 95/100, based on 744 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9/CRISPR-Cas9+crRNA/custom%40crispr-cas9-crrna%4042951897
Average 95 stars, based on 744 article reviews
crispr-cas9 crrna - by Bioz Stars,
2026-11
95/100 stars
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Expressing:Article Title: Glycine inhibits NINJ1 membrane clustering to suppress plasma membrane rupture in cell death Article Snippet: This allows for fusion of the insert and vector using the In-Fusion Snap Assembly EcoDry Cloning Kit (Takara, 638954) and transformation into Stellar competent bacterial cells (Takara). .. We used the same stock of iBMDMs from Incubation:Article Title: CRISPR/Cas9 mediated gene editing in non-model nematode Panagrolaimus sp. PS1159 Article Snippet: .. 1.25 μl tracrRNA (100 μM, catalog# 1073191, IDT) and x μM crRNA were mixed and incubated at 95°C for 5 min following incubation at RT for 5 min. 0.5 μl Sequencing:Article Title: Simultaneous measurement of the size and methylation of chromosome 4qA-D4Z4 repeats in facioscapulohumeral muscular dystrophy by long-read sequencing Article Snippet: AMII adapters were ligated to barcoded genomic DNA using Quick T4 DNA ligase (NEB, #E7185A) at room temperature for 10 min, followed by purification with AMPure XP Beads on a magnet. .. The DNA library from Synthesized:Article Title: Simultaneous measurement of the size and methylation of chromosome 4qA-D4Z4 repeats in facioscapulohumeral muscular dystrophy by long-read sequencing Article Snippet: AMII adapters were ligated to barcoded genomic DNA using Quick T4 DNA ligase (NEB, #E7185A) at room temperature for 10 min, followed by purification with AMPure XP Beads on a magnet. .. The DNA library from Knock-In:Article Title: Genome-scale mapping of DNA damage suppressors identifies GNB1L as essential for ATM and ATR biogenesis Article Snippet: FANCJ-KO and RECQL5-KO cell clones were identified by PCR amplification and ICE analysis ( https://ice.synthego.com ) and confirmed by immunoblot analysis (Supplementary Table 8). .. For the RPE1-hTERT TP53 −/− FANCJ knock-in cell lines, the desired FANCJ gene variants (K52R, K141/142A, S990A, T1133A) were introduced in the RPE1-hTERT TP53 −/− CRISPR:Article Title: Genome-scale mapping of DNA damage suppressors identifies GNB1L as essential for ATM and ATR biogenesis Article Snippet: FANCJ-KO and RECQL5-KO cell clones were identified by PCR amplification and ICE analysis ( https://ice.synthego.com ) and confirmed by immunoblot analysis (Supplementary Table 8). .. For the RPE1-hTERT TP53 −/− FANCJ knock-in cell lines, the desired FANCJ gene variants (K52R, K141/142A, S990A, T1133A) were introduced in the RPE1-hTERT TP53 −/− Article Title: Chromatin conformation dynamics during CD4+ T cell activation implicates autoimmune disease-associated genes and regulatory elements Article Snippet: .. |
![( A ) Volcano plot showing dysregulated genes in lung adenocarcinoma tumors (TCGA-LUAD cohort) carrying driver mutations in NFE2L2 or KEAP1 [cutoffs: Log2(FC) = ±0.6 and P -adj ≤ 0.05]. ( B ) Gene Set Enrichment Analysis (GSEA) using Kyoto Encyclopedia of Genes and Genomes (KEGG) gene sets in the TCGA-LUAD cohort with mutations in NFE2L2 or KEAP1 . ( C ) mRNA expression of selected genes involved in formaldehyde (FA) detoxification in KEAP1-NFE2L2 mutated and nonmutated (WT) LUAD tumors. ( D ) mRNA expression of selected genes in normal lung and whole blood obtained from GTEX. ( E ) Single-cell RNA-seq data from mouse hematopoietic and progenitor cells (HPC) plotted with kleintools ( https://kleintools.hms.harvard.edu/tools/spring.html ) . ( F ) Representative images of Western blots of selected factors from (A) performed in different cell lines. ( G ) Cystine uptake assay performed in selected cell lines (mean ± SEM, n = 3). ( H ) FA tolerance of A549 cells lacking SLC7A11 (sg SLC7A11 ) complemented with a wild-type copy of SLC7A11 (pSLC7A11) grown in the presence of 100 μM BSO (mean ± SEM, n = 3 and two-way ANOVA, **** P < 0.0001). ( I ) FA tolerance for H1299 cells harboring empty vector (pEV) or pSLC7A11 in the presence of 100 μM BSO [mean ± SEM, n = 3 and two-way ANOVA (Tukey test between untreated (UT) and BSO at each point); for H1299 pEV: **** P < 0.0001 and *** P = 0.0002]. ( J ) Viability of A549 cells carrying sg SLC7A11 and sgNTC (nontargeting control) under cystine deprivation [one-way ANOVA (Tukey test)]. ( K ) Glutathione (GSH) levels of the indicated cell lines maintained during 48 hours under cystine deprivation and exposing to FA during the last 24 hours [one-way ANOVA (Tukey test), ** P = 0.0058]. ( L ) FA tolerance of A549 cells lacking ADH5 in the presence of BSO (100 μM) or ERA (5 μM) [mean ± SEM, n = 6 and two-way ANOVA (Tukey test between UT and BSO or ERA at each point for multiple comparison); * P = 0.0300 and **** P < 0.0001].](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_3651/pmc13693651/pmc13693651__sciadv.aea1431-f2.jpg)